Journal: Cardiovascular Research

Article Title: Endothelium-specific SIRT7 targeting ameliorates pulmonary hypertension through Krüpple-like factor 4 deacetylation

doi: 10.1093/cvr/cvae011

Figure Lengend Snippet: Endothelial-specific loss of Sirt7 aggravates PH. ( A ) Immunofluorescence images of SIRT7 co-stained with CD31 in pulmonary arteries from lung tissues of IPAH patients ( n = 4) and control individuals ( n = 2). Scale bar = 20 µm. ( B ) Protein levels of SIRT7 in human PAECs isolated from IPAH patients ( n = 4) and control individuals (Ctrl, n = 4). ( C ) Western blotting analysis of Sirt7 protein expression in lung ECs isolated from Sirt7 f/f ; Cdh5 - Cre ERT mice intraperitoneally injected with TX (Tx, n = 6) or vehicle (Veh, n = 6). ( D–G ) Four-week-old Sirt7 f/f ; Cdh5 - Cre ERT mice were intraperitoneally injected with Tx or vehicle (Veh). Four weeks later, mice were exposed to normoxia (Nor) or SuHx for 4 weeks. RVSP ( D ) and Fulton Index ( E ) were measured. Vascularization of mice in various groups were shown by H&E staining ( F ) and angiography ( G ) of lung tissues. Scale bar = 20 µm ( F ) and 1 mm ( G ), respectively. Data are means ± SEM, and were analysed using Mann–Whitney U test ( B ), two-tailed Student t -test ( C ), and two-way ANOVA with Holm-Šídák's post-hoc test for multiple groups ( D–G ). * P < 0.05, ** P < 0.01, *** P < 0.001 with comparisons indicated by lines ( n = 6 mice per group).

Article Snippet: Human PAECs were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA) and cultured with endothelial culture medium (ECM; ScienCell Research Laboratories).

Techniques: Immunofluorescence, Staining, Control, Isolation, Western Blot, Expressing, Injection, MANN-WHITNEY, Two Tailed Test

Journal: Cardiovascular Research

Article Title: Endothelium-specific SIRT7 targeting ameliorates pulmonary hypertension through Krüpple-like factor 4 deacetylation

doi: 10.1093/cvr/cvae011

Figure Lengend Snippet: SIRT7 maintains PAEC function. ( A–F ) PAECs transfected with siRNA against SIRT7 (si S7 ) or negative control (siNC), or infected with lentivirus- SIRT7 ( S7 ) or control virus (Ctrl), were cultured in either 0.1% or 5% FBS for 48 h. Proliferation ( A , D ) and migration ( B , E ) were measured by EdU and wound healing assays, respectively. Tube formation analysis using PAECs transfected with siNC or si SIRT7 , or infected with Ctrl and lentivirus- SIRT7 for 48 h. ( C , F ) Quantified data shows the number of tubes and branch points for each group. Scale bar = 20 µm. ( G , I ) qPCR measurement of SIRT7 , TGFB1 , CTGF , cadherin 1 (ECAD) , IL-6 , IL-1B , NLRP3 , EDN1 , platelet derived growth factor subunit B (PDGFB) and BMPR2 in PAECs transfected with siNC or si SIRT7 , or infected with indicated lentivirus for 48 h. ( H ) Western blotting analysis of protein levels of SIRT7, TGF-β, eNOS, and α-SMA in PAECs transfected with siNC or si S7 for 48 h, and ( J ) protein levels of SIRT7, eNOS TGF-β, and BMPR2 in PAECs with or without SIRT7 overexpression for 48 h. Data are means ± SEM from 5 or 10 independent experiments, and were analysed by Kruskal–Wallis test with Dunn post-hoc test ( A , B , D , and E ) and Student t -test or Mann–Whitney U test ( C , F , G – J ), * P < 0.05, ** P < 0.01, *** P < 0.001 with comparisons indicated by lines.

Article Snippet: Human PAECs were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA) and cultured with endothelial culture medium (ECM; ScienCell Research Laboratories).

Techniques: Transfection, Negative Control, Infection, Control, Virus, Cell Culture, Migration, Derivative Assay, Western Blot, Over Expression, MANN-WHITNEY